For the quantification of curcuminoids, 6–7 ppm vinylic proton region in the 1H NMR spectrum was used, where acetone was observed as the suitable solvent in terms of curcuminoids solubility and proper resolution of peak. In the present work, sample preparation protocol is reported for the simultaneous determination of curcuminoids using 1H NMR.
Looking to improve productivity, time and simplicity, we are proposing a 1H NMR based approach for curcuminoids analysis and its applications to different geographical regions. When it comes to their analysis, most will rely on UV–Visible spectroscopy, HPLC and LC-MS methods. Curcumin, together with demethoxycurcumin and bisdemethoxycurcumin as a whole called curcuminoids, is an active phytochemical constituent present in the turmeric.
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